Totect Study guides, Class notes & Summaries

Histotechnology - FixationThe primary purpose of fixation is to: a. stop enzymatic action b. remove the water from the tissue c. provide support for the tissue d. autolyze the tissue - correct answers a Which of the following fixatives contains picric acid, formalin, and acetic acid? a. Zenker b. Helly c. Bouin d. Zamboni - correct answers c The volume of fixative should exceed the volume of the tissue by: a. 1 time to 2 times b. 5 times to 10 times c. 15 times to 20 ...

Receiving Gross examination Fixation Decalcification Dehydration Clearing Infiltration Embedding Sectioning Staining Mounting CORRECT ANSWER-11 routine procedures in the histopathology laboratory clue: RG - FD - DC - IE - SSM Check the label CORRECT ANSWER-When receiving the specimen, make sure to-- Patient's name Age Sex Relevant clinical data Surgical findings Nature of operation Name of the tissue submitted CORRECT AN

Histotechnology: A Self-Instructional Text, 4th edFor adequate fixation, the fixative volume should be at least ____ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 - correct answers 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope - correct answers Polarizing microscope For most routine H & E...

Histotechnology: H&E Staining the study of the microscopic structure of tissues what is histology? 1. to determine the cause of disease 2. determine the mechanism by which disease develops 3. to see the structural alterations of cells 4. to determine the consequences of the cell's alteration what are 4 ways a pathologist can use histology? so we can see all the components of the cell and the relation of cells to one another why do we stain tissues on slides? to differentia...

Histotechnology- Staining Questions (HW Questions)Dyes may be classified as natural or synthetic. Examples of natural dyes are: - correct answers hematoxylin, carmine, and indigo Mordants are generally classified as: - correct answers Metals Generally, acid dyes are differentiated in solutions that are weakly: - correct answers basic A dye in which the coloring elements are found in the anionic portion of the compound is a/an: - correct answers acid dye Differential staining of nucle...

HTL Histotechnology Exam 1The overall magnification of a microscope is achieved by what two values? - correct answers objective lens x eyepiece lens What is the magnification of scanning or low power? - correct answers 2.5x to 4.0x What is the magnification of intermediate power? - correct answers 10x to 20x What is the magnification of high power? - correct answers 40x What is the magnification of high dry? - correct answers 100x What is the magnification of oil immersion? - corr...

histotechnology test 2affinity or avidity: a dye is attracted to a tissue - correct answers affinity affinity or avidity: how strongly a dye is attracted to a tissue - correct answers avidity what happens when amphoteric tissue (or dye) is placed in an acidic solvent? - correct answers becomes basic what happens when amphoteric tissue (or dye) is placed in a basic solvent? - correct answers becomes acidic what defines "how fast a dye stains" - correct answers vigour what type of...

Alcian Blue demonstrates - Answer Acid mucins ATPase Enyme Histochemistry demonstrates - Answer ATPase in muscles Bielschowsky's Silver Technique demonatrates - Answer Dendrites, axons, neurofibrils Brown & Brenn gram stain demonstrates - Answer bacteria Congo red demonstrates - Answer amyloid Cresyl Echt Violet demonstrates - Answer Nissl substance Geimsa stain demonstrates - Answer mast cells or H. pylori Gomori Burtner demonstrates - Answer melani...

HTL Histotechnology Exam 4List types of carbohydrates? - correct answers - sugars - starch - Cellulose - polymers linked to proteins - glycogen - Group I Neutral polysaccharide - Group II Acid Muco Polysaccharide - Group III Glycoprotein - Group IV Glycolipid What is a Group I Carbohydrate that is glucose which contains glycogen, starch, and cellulose? - correct answers Neutral Polysaccharide What is Group II Carbohydrate? - correct answers Acid Muco polysaccharide What is Grou...

What is the purpose of embedding? CORRECT ANSWER-hold tissue; orient tissue Why do we want the tissue held? CORRECT ANSWER-so we can cut very thin sections and it has more stability Why do we orient tissue? CORRECT ANSWER-so that the view the pathologist has is optimal for diagnosis List the two types of Embedding media CORRECT ANSWER-Water-soluble and water-insoluble List examples of water-soluble media CORRECT ANSWER-Carbowax, gelatin, agar, OCT What is carbowax used for? CORREC...