Totect Study guides, Class notes & Summaries

Absorption - correct answers When tissue is penetrated by, or absorbs, a dye solution and becomes colored without any other change or chemical reaction occurring. An example is the absorption of oil red O or Sudan black B by fat Achromatic - correct answers Without color, or not easily stained. In microscopy, achromatic lenses are corrected for two colors, red and blue. This helps produce images free of chromatic aberrations Acid mucosubstances - correct answers a term used to include bot...

Microscope - correct answers Used to for the magnification of the tissues and cells. Microtome - correct answers Used for the cutting of the wax and tissues. Block holder (chuck) - correct answers Holds the tissue in position. Knife and Knife carrier - correct answers For actual cutting of tissue sections. Pawl, Ratcher Feed Wheel & Adjustment screw - correct answers Lines up the tissue block in proper position with knife, adjusting thickness of tissues for successive sections. So...

For adequate fixation, the fixative volume should be at least ______ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 CORRECT ANSWER-D. 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid. A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope CORRECT ANSWER-B. Polarizing microscope For most routine H & E staining, the sections should be 1 cel...

What method is used to stabilize proteins making it resistant to further damage? CORRECT ANSWER-Fixation Name two methods of fixation? CORRECT ANSWER-Chemical reaction (formaldehyde) and physical (heat or dessication) What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** CORRECT ANSWER-bacteria or mold What factor causes autolysis if tissue is not fixed? **** CORRECT ANSWER-enzyme attack What are four functions of fixatives? CORRECT ANSWER-1. kills tissue ...

What are the steps of processing? CORRECT ANSWER-Fixation, (decalcification), dehydration, clearing, infiltration, embedding The step of processing removes water for the next subsequent step to occur (xylene and paraffin) CORRECT ANSWER-dehydration What accounts for the majority of tissue processing problems? **** CORRECT ANSWER-incomplete dehydration How does one solve incomplete dehydration? CORRECT ANSWER-Reprocess in water and then absolute alcohol. Or melt paraffin block, pat tissu...

Histotechnology HTL Exam - Comprehensive Review questions with correct answers

Histotechnology - NSH- Gross Dissection and Description at the Basic Level questions with correct answers

Acetic acid CORRECT ANSWER-This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde CORRECT ANSWER-Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. Glyoxal CORRECT ANSWER-This fixative is the smallest ...

Histotechnology Mid TermWhen is H&E QC done? - correct answers -Daily -Before any patient slides are stained What is H&E QC? - correct answers -Slides are checked manually for approval -Documented and logged for future inspections What is the meniscus? - correct answers -Bottom line of the curved surface of the liquid How many grams are needed to make 500mL of the 10% solution? - correct answers % desired:100 = g needed:volume desired -50g What causes tissue to shrink, dry, brit...

1. If there is venetian blind pattern in the section, a possible remedy is to: CORRECT ANSWER-Tighten all screws 2. To decrease the effect of static electricity: CORRECT ANSWER-ground microtome or breathe on it 3. To remedy crooked ribbon formation: CORRECT ANSWER-trim block edges parallel 4. If the specimen is mushy-appearing and sectioning is difficult: CORRECT ANSWER-a. melt block, reprocess & re-embed 6. A scratching noise during sectioning can be caused by: CORRECT ANSWER-tissue...